CPT chubu 8ml
A Vacutainer CPT (Cell Preparation Tube) 8mL ndi chubu chapadera chotolera magazi chomwe chimagwiritsidwa ntchito popatula ma cell a mononuclear (PBMCs) m'magazi athunthu. Amagwiritsidwa ntchito kwambiri mu immunology, kafukufuku wa matenda opatsirana, komanso ntchito zachipatala monga kuwunika kwa chitetezo chamthupi komanso kuyesa kwa ma cell.
Zofunika Kwambiri za Vacutainer CPT Tube (8mL):
Kudzazidwa ndi anticoagulant ndi kachulukidwe gradient sing'anga
Muli sodium citrate ngati anticoagulant.
Mulinso gel olekanitsa a Ficoll™-based density gradient kuti athandizire kulekanitsa ma cell a mononuclear.
Imayatsa gawo limodzi la PBMC kudzipatula
Imathetsa kufunikira kwa kachulukidwe kachulukidwe ka gradient centrifugation (mwachitsanzo, Ficoll-Paque™).
Amachepetsa nthawi yokonza ndi kusinthasintha.
Kuchuluka kwa magazi okhazikika
Amapangidwa kuti azipanga mpaka 8mL yamagazi athunthu.
Wosabala, wogwiritsa ntchito kamodzi
Amagwiritsidwa ntchito pazachipatala ndi kafukufuku komwe kudzipatula kwapamwamba kwa PBMC kumafunikira.
Yogwirizana ndi centrifugation
Njira yodziwika bwino ya centrifugation ndiyofunikira kuti tisiyanitse bwino ma PBMC ndi zigawo zina za magazi.
Kagwiritsidwe ndi Ntchito:
Kudzipatula kwa PBMC pamaphunziro a chitetezo chamthupi (ma cell T, B cell, NK cell, monocytes).
Flow cytometry, ELISPOT, kuyesa kwa cytokine, ndi kusanthula kwina kwa immunological.
Kafukufuku wa matenda opatsirana komanso katemera.
Kuwunika kwa matenda a Autoimmune.
Mayesero azachipatala ndi kupezeka kwa biomarker.
Momwe Imagwirira Ntchito (Njira Yoyambira):
1. Sungani magazi mu chubu cha CPT (dzazani ku voliyumu yomwe mwasankha).
2. Sinthani pang'onopang'ono (nthawi 8-10) kusakaniza ndi anticoagulant.
3. Centrifuge malinga ndi ndondomeko ya wopanga (nthawi zambiri kuzungulira 1500-1800 RCF kwa mphindi 20-30 kutentha kwapakati).
4. PBMC wosanjikiza mafomu pamwamba pa olekanitsa gel osakaniza pambuyo centrifugation.
5. Chotsani mosamala PBMC wosanjikiza pogwiritsa ntchito pipette kwa mapulogalamu apansi.
Kusunga ndi Kusamalira:
Sungani kutentha kwapakati (15-25 ° C).
Pewani kutentha kwambiri kapena kuzizira kwambiri.
